Vaccine Preparation and Quality Control of Killed Leishmania major

preparation of killed leishmania vaccine and its evaluation for control of visceral leishmaniasis in dogs

in this study, 16 eligible dogs were randomly divided in to 4 groups. group 1, 2, 3 and 4 received autoclaved leishmania infantum with bcg (ali+bcg), autoclaved leishmania major + bcg (alm + bcg), bcg alone and normal saline, respectively. dogs received the vaccines intradermally 3 times at 30 days intervals. all dogs were followed up 2 months intervals by leishmanin skin test and anti -leishma...

Immuno-Biochemical Alterations in Leishmania major Infected Balb/c Mice after Immunization with Killed Leishmania Vaccine and BCG as Adjuvant

BCG is an immune modulatory that inducing humoral and cellular immune responses during a number of infections including cutaneous leishmaniasis. Killed Leishmania Vaccine (KLV) has been applied for its immunogenicity in hosts. This study was carried out between 2010 and 2011 at the Department of Parasitology, Pasteur Institute of Iran. In the present study, alterations of Nitric Oxide (NO) leve...

Use of Sodium Alginate Adjuvant in Preparation of a Formalin Killed Vaccine Against Infectious Bovine Rhinotracheitis

Randomised vaccine trial of single dose of killed Leishmania major plus BCG against anthroponotic cutaneous leishmaniasis in Bam, Iran.

BACKGROUND A vaccine consisting of a single dose of whole-cell autoclave-killed Leishmania major (ALM) mixed with BCG was assessed in comparison with BCG alone against anthroponotic (human to human transmission) cutaneous leishmaniasis in a randomised double-blind trial in Bam, Iran. METHODS 3637 schoolchildren, aged 6-15 years, with no history of cutaneous leishmaniasis and no response to a ...

Safety and immunogenicity of an autoclaved Leishmania major vaccine.

OBJECTIVE To test the safety and immunogenicity of two doses of autoclaved L. major (ALM) vaccine mixed with BCG. SETTING Kala-azar endemic area of eastern Sudan. DESIGN This was a randomised, double blind and BCG controlled phase I/II study. SUBJECTS Eighty healthy volunteers (forty children and forty adults) with no past history of kala-azar, no reactivity to leishmanin antigen and with...

PLGA Nanospheres Loaded with Autoclaved Leishmania Major (ALM) and CpG-ODN: Preparation and in vitro Characterization

Objective(s) Several antigens, adjuvants and delivery systems have been evaluated for induction of protective immune responses against Leishmaniasis, but most of them have been inefficient. In this study, PLGA nanospheres as antigen delivery system CpG-ODN as an immunoadjuvant for increasing the immune responses against Autoclaved Leishmania major (ALM) were prepared and characterized. Materi...